Skip to main content
. 2020 Mar 18;117(13):7317–7325. doi: 10.1073/pnas.1914830117

Fig. 5.

Fig. 5.

Pyoverdine is required for P. aeruginosa virulence against wild-type but not Tsf1JP94 flies. (A and B) Survival rates of wild-type and Tsf1JP94 mutant flies infected with (A) wild-type, pyochelin-deficient pchE or (B) pyoverdine-deficient pvdP P. aeruginosa PA14. (C) Measurement of wild-type and pvdP PA14 load 16 h after infection of wild-type and Tsf1JP94 flies. Each dot represents cfus from a pool of five animals, calculated per fly. The mean and SD are shown. (D) Survival rates of wild-type and Tsf1JP94 flies preinjected with 13.4 nL of H2O (control) or with 13.4 nL of 100 µM repressor of pyoverdine flucytosine prior to infection with P. aeruginosa. (E and F) Survival rates of wild-type and Tsf1JP94 flies infected with wild-type, (E) ΔpchD, ΔpvdA, and (F) ΔTonB1 P. aeruginosa PAO1. Survival graphs show one representative experiment out of three independent experiments with similar results with two or three cohorts of 20 male flies per treatment. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, nonsignificant, P > 0.05.