Fig. 6.

Tsf1 contributes to intestinal immunity. (A and B) Tsf1 expression in wild-type and Rel^E20 guts after (A) Ecc15 and (B) Pe infection, measured by RT-qPCR (n = 20 guts per group). The mean and SD of three independent experiments are shown. (C) Survival rates of wild-type, Rel^E20, and Tsf1^JP94 flies orally infected with P. entomophila. (D) Survival rates of flies with gut-specific (Myo1A) knockdown of Tsf1 are significantly reduced compared to wild-type flies after Pe oral infection. (E) Increased susceptibility of Tsf1^JP94 mutant flies to Pe oral infection is rescued by gut-specific overexpression of the wild-type (UAS-Tsf1^WT) but not mutated form of Tsf1 (UAS-Tsf1^{Fe mutant}). (F) Survival rates of wild-type and Tsf1^JP94 flies orally infected with wild-type, pchE, and pvdP P. aeruginosa PA14. Survival graphs show one representative experiment out of three independent experiments with similar results with two to three cohorts of 20 flies per treatment. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, nonsignificant, P > 0.05.