Fig. 2.
Knockout of COST1 confers drought tolerance and induces the expression of a spectrum of stress-responsive genes. (A and B) WT, cost1 (cost1-1), gCOST1#1 (complementation line with COST1 genomic DNA), and two COST1 RNAi lines (COST1-RNAi#1 and COST1-RNAi#3) were subjected to drought treatment for 2 wk, and the water loss of detached rosette leaves from each genotype was recorded every 30 min for 5 h (C). (D) Expression of representative drought responsive genes in WT and cost1 with and without drought treatment was assessed by qPCR. (E) Clustering of DEGs (fold-change in expression level >2 between two samples) in WT and cost1 plants with and without dehydration treatment, “C” denotes control and “D” denotes dehydration. Color legend denotes normalized gene-expression value. (F and G) Comparison of drought-regulated DEGs in whole-transcriptome RNA-seq. (H and I) Enrichment of Biological Process GOs in drought-regulated DEGs. Color scale: −log10 (adjusted P value). Over- and underrepresentation are shown in red and blue, respectively.