Skip to main content
. 2020 Mar 13;117(13):7482–7493. doi: 10.1073/pnas.1918539117

Fig. 3.

Fig. 3.

Expression of COST1 during drought stress. (A) Localization and characteristics of COST1-YFP protein after 3 h of dehydration treatment. (Scale bar, 10 µm.) (B and C) Quantification of fluorescence intensity and punctate structures before and after 3 h of dehydration treatment. Signals were quantified for at least 10 images per replicate, with 3 biological replicates. Asterisks indicate significant differences, compared with no treatment. (D) Immunoblot of COST1-YFP protein after dehydration treatment for the indicated times using antibodies against GFP. The number below indicates the band intensity of COST1-YFP, and Ponceau staining was used as a loading control. (E) Immunoblot of COST1-YFP protein after treatment with DMSO (control), MG132, or ConcA. (F) COST1-YFP was immunoprecipitated after treatment with DMSO, MG132, or ConcA under normal conditions, followed by detection using anti-GFP antibodies. (G) Ubiquitination of COST1-YFP after dehydration of 10-d-old COST1-YFP or YFP transgenic plants for 6 h. After immunoprecipitation with GFP-trap, samples were immunoblotted using antibodies against ubiquitin. Transgenic COST1-YFP plants were generated in cost1 mutant background with full complementation.