Fig. 4.

AtaC hydrolyzes c-di-AMP in vivo and is expressed during the life cycle of S. venezuelae. (A) Intracellular c-di-AMP levels in S. venezuelae wild type and ΔataC during late vegetative growth (10 to 12 h), early sporulation (14 to 16 h), and sporulation (18 to 20 h). Data are presented as the mean of biological replicates ± SD (n = 3). (B) Expression profile of AtaC in S. venezuelae wild type grown in a liquid sporulation medium (MYM). AtaC was detected using a polyclonal anti-AtaC antiserum. Protein samples harvested from ΔataC served as negative control. Purified 6xHis-AtaC served as the positive control.