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. Author manuscript; available in PMC: 2021 Feb 4.
Published in final edited form as: Biochemistry. 2020 Jan 7;59(4):615–626. doi: 10.1021/acs.biochem.9b01026

Figure 5.

Figure 5.

WecA UDP-GlcNAc transferase activity with fl-BPs. Cef from indicated strains was incubated with either 2CN (A) or NBD-B(4Z)P (B) and UDP-GlcNAc as the donor sugar. The cef C43 forms fl-BPP-HexNAc at a retention time of 3.0 and 3.4 for 2CN and NBD, respectively, when co-incubated with UDP-GlcNAc. Adding 1 μg/mL of tunicamycin, designated by (*), eliminates transferase activity. An additional fluorescence peak (•) is observed at 6.2 and 6.4 min for 2CN and NBD respectively. A co-eluting contaminate is present in the 2CN-B(4Z)P standard, which appears at 10 min only after BP consumption.