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Phosphohexosetransferase activity with fl-BPs. Formation of fl-BPP-Hexose appears at a retention time of 3.0 and 3.4 for 2CN (A) and NBD (B), respectively, when co-incubated with their respective UPD-linked sugar donor. NBD-B(4Z)P does not appear to be a substrate for WbaP. An additional fluorescence peak (•) is observed at 6.2 and 6.4 min for 2CN and NBD respectively.
