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WbaP catalyzes the transfer of UDP-Glc to fl-BPs. Promiscuous transferase activity is demonstrated with cholate and 2CN (A) or 5% n-propanol supplementation and NBD (B). Fl-BPP-Glc is observed at 3.0 and 3.7 min for 2CN and NBD, respectively. A prominent unanticipated fluorescence peak (•) is observed at 6.3 min for 2CN only.
