Figure 1. Regulation of circRNA‐FNTA expression and function in response to androgen receptor (AR) in bladder cancer (BCa) cells.

• A
  The expression of AR was determined using Western blots (overexpressing AR in J82 cells and knocking down AR in UMUC3 cells).
• B
  Relative expression of the top 20 up‐regulated circRNAs from the GEO dataset GSE92675 (BCa tumor tissues compared to para‐tumor tissues) in response to AR modulation in J82 and UMUC3 cells. The # indicates an RNA with a relatively low expression (qRT–PCR cycle number > 35); thus, the circRNA was excluded for further analysis.
• C
  Schematic illustration showing the 4 circRNAs identified that could be regulated by AR.
• D
  Schematic illustration showing the position of the targeting shRNA to knock down the circRNAs (sh‐circ) by targeting the specific 5′–3′ splice junction.
• E
  Real‐time quantitative PCR (qRT–PCR) results show the knockdown efficiency for these 4 circRNAs in J82 oe‐AR (J82‐AR) cells.
• F
  Transwell invasion assays showed that knockdown of hsa_circ_0084171 (circ_0084171), but not the other circRNAs, can partly reverse oeAR‐increased invasion in J82 cells. Scale bar, 100 μm.
• G
  MTT assays showed that shcirc_0084171 can partly reverse oeAR‐increased cisplatin chemo‐resistance (2 μg/ml) in J82 cells. To calculate the mean, the value of 0 μg/ml cisplatin groups was set as 1.
• H
  The qRT–PCR results showed the overexpression (oe) efficacy for hsa_circ_0084171 (oecirc_0084171) in UMUC3‐shAR cells.
• I
  Transwell invasion assays showed that oecirc_0084171 can partly rescue shAR‐induced invasion reduction in UMUC3 cells. Scale bar, 100 μm.
• J
  MTT assays showed that oecirc_0084171 can partly overcome shAR effect for cisplatin chemo‐resistance (2 μg/ml) in UMUC3 cells.

Data information: (B, E, F, G, H, I, and J) Data shown represent the means ± SD (n = 3 biological replicates). Student's t‐test was used for the statistical analysis (*P < 0.05, **P < 0.01, ***P < 0.001).Source data are available online for this figure.