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. 2020 Mar 4;21(4):e49269. doi: 10.15252/embr.201949269

Figure EV2. WDR63 is upregulated by p53.

Figure EV2

  • A
    A549 cells expressing control shRNA, WDR63 shRNA#1, or WDR63 shRNA#2 (each also expressing luciferase) were injected via tail vein into nude mice (n = 6 for each group). Six weeks after injection, tumor formation in lungs was monitored by bioluminescence imaging. Data shown are mean ± SD; *P < 0.05; one‐way ANOVA. Scale bar: 1 cm.
  • B
    WDR63 expression levels were analyzed in TCGA lung adenocarcinoma (LUAD) and their normal tissues. Data shown are mean ± SD; P‐value was calculated by two‐tailed Student's t‐test.
  • C
    WDR63 expression levels were analyzed in TCGA lung squamous cell carcinoma (LUSC) and their normal tissues. Data shown are mean ± SD; P‐value was calculated by two‐tailed Student's t‐test.
  • D
    WDR63 expression levels were analyzed in TCGA LUAD with different stages. Data shown are mean ± SD; P‐value was calculated by one‐way ANOVA.
  • E
    WDR63 expression levels were analyzed in TCGA LUSC with different stages. Data shown are mean ± SD; P‐value was calculated by one‐way ANOVA.
  • F
    A549 cells expressing control or p53 shRNA were treated with etoposide (50 μM) for the indicated periods of time. WDR63 mRNA and protein levels were then examined. Data shown are mean ± SD; n = 3 independent experiments. ***P < 0.001; two‐tailed Student's t‐test.
  • G
    A549 cells expressing control or p53 shRNA were treated with Nutlin (10 μM) for the indicated periods of time, followed by Western blot analysis. Data shown represent three independent experiments.
  • H
    HCT116 p53 +/+ and HCT116 p53 −/− cells were treated with Nutlin (10 μM) for the indicated periods of time, followed by Western blot analysis. Data shown represent three independent experiments.
  • I
    WDR63 expression levels were analyzed in TCGA LUAD harboring wild‐type or mutant TP53 gene using TCGA database. Data shown are mean ± SD; P‐value was calculated by two‐tailed Student's t‐test.
  • J
    WDR63 expression levels were analyzed in TCGA LUSC harboring wild‐type or mutant TP53 gene using TCGA database. Data shown are mean ± SD; P‐value was calculated by two‐tailed Student's t‐test.
  • K
    A549 cells expressing either control shRNA or p53 shRNA were transfected with the indicated reporter constructs plus Renilla luciferase plasmid. Twenty‐four hours later, reporter activity was measured. Data shown are mean ± SD; n = 3 independent experiments. **P < 0.01; ***P < 0.001; two‐tailed Student's t‐test.