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A
Lysates from HEK293T cells expressing GFP‐WDR63 alone or GFP‐WDR63 plus Flag‐tagged Arp2, Arp3, ArpC2, and coronin 1B as indicated were immunoprecipitated by anti‐Flag antibody, followed by Western blot analysis. Data shown represent three independent experiments.
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B
Lysates from HEK293T cells expressing GFP‐WDR63 alone or GFP‐WDR63 plus Flag‐tagged cortactin, N‐WASP, and cofilin as indicated were immunoprecipitated by anti‐Flag antibody, followed by Western blot analysis. Data shown represent three independent experiments.
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C
Lysates from A549 cells were incubated with purified GST or GST‐WDR63 proteins immobilized on glutathione beads. Input and bead‐bound proteins were analyzed by Western blot. Data shown represent three independent experiments.
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D
The cellular localization of exogenous GFP‐WDR63 was determined by immunofluorescence. Data shown represent three independent experiments. Scale bar: 20 μm.
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E
The localization of endogenous WDR63 in A549 cells was evaluated by nuclear/cytosolic fractionation, followed by Western blot analysis. Data shown represent three independent experiments.
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F
A549 cells expressing Flag‐Arp2 alone or together with HA‐WDR63 were serum‐starved for 6 h. Cells were then treated with or without 10% FBS for 30 min, followed by immunofluorescence assay. Data shown represent three independent experiments. Scale bar: 20 μm.
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G
Lysates from HEK293T cells expressing GFP‐WDR63 alone or together with Flag‐VCA were immunoprecipitated by anti‐Flag antibody, followed by Western blot analysis. Data shown represent three independent experiments.
