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. 2020 Mar 4;21(4):e49269. doi: 10.15252/embr.201949269

Figure EV5. WDR63 mediates the metastasis‐suppressive function of p53.

Figure EV5

  • A–D
    H292 cells expressing control shRNA, p53 shRNA, or p53 shRNA plus Flag‐WDR63 were subjected to wound‐healing (A), transwell migration (C), and transwell invasion (D) assays. Lysates from these cells were also analyzed by Western blot (B). The shown images are representative of three independent experiments. Data shown are mean ± SD (n = 3). *P < 0.05; **P < 0.01; one‐way ANOVA. Scale bar in (A): 200 μm. Scale bars in (C and D): 100 μm.
  • E, F
    A549 cells expressing either control shRNA or p53 shRNA were infected with lentiviruses expressing Flag‐tagged full‐length or truncated WDR63 proteins as indicated. Cells were then subjected to wound‐healing assay (E). Lysates from these cells were also analyzed by Western blot (F). The shown images are representative of three independent experiments. Data shown are mean ± SD (n = 3). *P < 0.05; **P < 0.01; one‐way ANOVA. Scale bar: 200 μm.
  • G
    A549 cells expressing control shRNA, p53 shRNA, or p53 shRNA plus Flag‐WDR63 (each also expressing luciferase) were injected via tail vein into nude mice (n = 6 for each group). Six weeks after injection, tumor formation in lungs was monitored by bioluminescence imaging. Data shown are mean ± SD; *P < 0.05; one‐way ANOVA. Scale bar: 1 cm.