Figure EV1. Physical interaction of Gal‐3 and Gal‐1 with CC and CXC chemokines.

• A–D
  Chemokines were immobilized on a nitrocellulose membrane and incubated with (A) TBS or (B) TBS containing biotinylated Gal‐1. The membranes were then stepwise incubated in solutions with SA‐HRP and chemiluminescence reagents. (C, D) The blots were subjected to densitometric analysis.
• E–G
  Binding of galectins to chemokines was further assessed by immobilizing (E) Gal‐3, (F) Gal‐3 CRD, and (G) Gal‐1 on sensor chips at a density of 700 RU and detecting signals of human chemokines under flow. Signals of the solid‐phase assays (Fig 1E) are depicted in light blue (n = 3). Data represent mean ± SD from three independent experiments.