Figure 3. Gal‐3‐mediated inhibition of CXCL12‐induced leukocyte migration in vitro .

• A–C
  Jurkat T cells migrated in the presence of 10 nM CXCL12 with increasing concentrations of (A) Gal‐3, (B) Gal‐3 CRD, and (C) Gal‐1 (A–C: n = 4).
• D
  Human CD4⁺ T cells migrated in the presence of 10 nM CXCL12 alone and with 10 nM Gal‐3 (dark green), Gal‐3 CRD (light green), and Gal‐1 (blue, n = 3).
• E, F
  (E) Jurkat T cells migrated to increasing concentrations of CXCL12 alone (black) and CXCL12 in the presence of 0.1 nM of Gal‐3 CRD (light green). (F) The inhibitory effect of Gal‐3 CRD is shown as percentage of the chemotactic effect of CXCL12 (E, F: n = 3).
• G
  Jurkat T cells were allowed to migrate in the presence of CXCL12 at increasing concentrations alone and with 0.1 nM Gal‐1 or 100 nM Gal‐1 (n = 3).
• H
  Jurkat T cells did not migrate in the sole presence of 1 nM to 1 μM Gal‐3, Gal‐3 CRD (both n = 5), or Gal‐1 (n = 4).
• I
  The viability of Jurkat T cells was assessed after incubation with 1 μM CXCL12 alone and in the presence of Gal‐3, Gal‐3 CRD, and Gal‐1 (n = 2).
• J, K
  (J) THP‐1 cells and (K) neutrophils migrated in the presence of 10 nM CXCL12 alone and with 10 nM Gal‐3, Gal‐3 CRD, and Gal‐1 (J, K: n = 3).
• L
  Human eosinophils migrated in the presence of 10 nM CCL26 alone and with 10 nM Gal‐3, Gal‐3 CRD, and Gal‐1 (n = 3).
• M
  CD4⁺ T cells migrated in the presence of 1 nM CCL17 alone and with equimolar concentrations of Gal‐3, Gal‐3 CRD, and Gal‐1 (n = 4).

Data information: Cell migration is shown as absolute cell counts. Data represent the mean ± SD from the indicated number of independent experiments each performed in three technical replicates, and these were statistically analyzed by using (A–C, F–G, J) a single sample t‐test or (D, I, J–M) by an unpaired t‐test against the effect of the chemokine alone or as indicated (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).