Figure EV3. 1H–15N chemical shift maps for 15N‐labeled CXCL12 with WT Gal‐3 CRD and its mutants.
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A–CΔδ Values plotted vs. the amino acid sequence of CXCL12 are shown for 30 μM 15N‐enriched CXCL12 in the presence of 500 μM label‐free Gal‐3 CRD mutants (A) Q220E, (B) Q220K, and (C) H217A (black). The results of the experiment with WT Gal‐3 CRD from Fig 2A are overlaid in gray.