Figure 4. Peritoneal recruitment of leukocytes by CXCL12 in the presence of Gal‐3.

• A, B
  The peritoneal recruitment of (A) CD45⁺/CD115⁻/Ly6G⁺ neutrophils and (B) CD45⁺/CD115⁺/Ly6C^hi classical monocytes in C57BL/6J mice was assessed 4 h after intraperitoneal (IP) injection of 500 nM CXCL12 alone (black) and in the presence of 50 nM Gal‐3 (dark green; A, B: n = 7 mice).
• C
  The peritoneal recruitment of classical monocytes after IP injection of PBS (n = 6) or TG in C57BL/6J WT (dark green, n = 10 mice) and Gal‐3^−/− (black, n = 5 mice) mice was assessed after 18 h. Where indicated, the mice received an IP injection of CXCR4 antagonist AMD 3465 12 h prior to the experiment.
• D
  The concentration of CXCL12 concentration was determined by ELISA on the peritoneal lavage normalized with levels from the mesenterium (n = 4 mice).
• E
  CXCR4 expression levels on Ly6C^hi monocytes of the blood and the peritoneal lavage after 18 h of TG stimulation were determined by flow cytometry and indicated as mean fluorescence intensity (MFI) (n = 4 mice).

Data information: Cell migration to the peritoneum is shown as cells/ml lavage. Data represent the mean ± SD from the indicated number of mice and were statistically analyzed by using the unpaired t‐test, as indicated (*P ≤ 0.05, **P ≤ 0.01).