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. 2020 Feb 21;21(4):e47852. doi: 10.15252/embr.201947852

Figure EV4. Co‐localization of CXCL12 and Gal‐3 on cells in vivo .

Figure EV4

  • A, B
    Proximity of Gal‐3 and CXCL12 on cells from peritoneal lavages after injection of (A) PBS or (B) TG was determined by PLA (representative example of n = 3). White scale bar: 10 μm.
  • C, D
    Co‐localization of Gal‐3 and CXCL12 in frozen sections of lymph nodes from (C) WT and (D) CXCL12−/− mice was determined by immunofluorescence staining using antibodies against Gal‐3 and CXCL12 (representative example of n = 3). Scale bar: 40 μm. Arrows indicate subcapsular sinus macrophages (SSM).
  • E
    Co‐localization on the same sections was also detected by PLA (representative example of n = 3). Scale bar: 40 μm.