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. 2020 Feb 27;21(4):e49075. doi: 10.15252/embr.201949075

Figure EV5. CriptoMy‐ LOF affects endothelial plasticity in injured skeletal muscle.

Figure EV5

  • A
    Representative confocal pictures of double immunostaining with CD31 (red) and PDGFRα (green) on Control and CriptoMy‐LOF TA sections at day 5 after injury. Split green and red channels of Fig 7E are shown.
  • B
    Representative pictures of triple immunostaining with VEcad (red), KLF4 (green), and pSMAD3 (white) on Control and CriptoMy‐LOF TA sections at day 5 after injury. Merged channels of VEcad/KLF4 (top panels) and VEcad/pSMAD3 of Fig 7H are shown.
  • C, D
    Representative pictures of double immunostaining with VEcad (red) and KLF4 (green) on Control and CriptoMy‐LOF TA sections at day 2 (C, left panels) and day 3 (D, left panels) after injury and quantification of VEcad/KLF4 double‐positive cells per area (mm2) at day 2 (C, right panel) and day 3 (D, right panel).
Data information: Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. Confocal pictures (A) scale bar: 25 μm. Magnification of the boxes is 3.5×. Data are expressed as box plots displaying minimum, first quartile, median, third quartile, and maximum (n ≥ 5 biological replicates; P = ns; Student's t‐test).