Figure 7. Cripto modulates endothelial plasticity by restricting Endothelial‐to‐Mesenchymal Transition.

• A
  Representative pictures of double immunostaining with VEcad (red; left and middle panels) and KLF4 (green, left panel) or TCF4 (green, middle panel), and with CD31 (red; right panels, confocal pictures) and TCF4 (white, right panel) on Control and Cripto^My‐LOF TA sections at day 5 after injury.
• B–D
  Quantification of VEcad/KLF4 (B), VEcad/TCF4 (C), and CD31/TCF4 (D) double‐positive cells per area (mm²) in Control and Cripto^My‐LOF TA sections at day 5 after injury.
• E
  Representative Confocal pictures of CD31⁺ (red) and PDGFRα⁺ (green) immunostaining on Control and Cripto^My‐LOF TA sections at day 5 after injury.
• F, G
  Quantification of CD31⁺/PDGFRα⁺ (F) and CD31⁻/PDGFRα⁺ (G) cells per area (mm²).
• H, I
  Representative pictures of triple immunostaining with VEcad (red), KLF4 (green), and pSMAD3 (white) on Control and Cripto^My‐LOF TA sections at day 5 after injury (H) and quantification of VEcad/KLF4/pSMAD3 triple‐positive cells per area (mm²; I).
• J, K
  Representative pictures of double immunostaining with VEcad (red) and KLF4 (green) on Control and Cripto^My‐LOF TA sections at day 5 after re‐injury (CTX‐II; J) and on diaphragm sections of mdx‐Control and mdx‐Cripto^My‐LOF (K).
• L, M
  Quantification of VEcad/KLF4 double‐positive cells per area (mm²) in Control and Cripto^My‐LOF TA sections at day 5 after re‐injury (CTX‐II; L) and in diaphragm sections of mdx‐Control and mdx‐Cripto^My‐LOF (M).

Data information: Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. Confocal pictures scale bar: 25 μm. Magnification of the boxes is 3.5×. Data are expressed as box plots displaying minimum, first quartile, median, third quartile, and maximum (n ≥ 5 biological replicates; *P < 0.05; **P < 0.01; ***P < 0.001; Student's t‐test).