Skip to main content
. 2020 Feb 21;21(4):e49115. doi: 10.15252/embr.201949115

Figure EV3. Transplantation efficiency, survival, and proliferation of TGF‐β1‐silenced or miR‐21‐silenced AT‐MSCs in CD18−/− wounds.

Figure EV3

  • A
    TGF‐β1‐siRNA‐transfected MSCs or miR21 inhibitor‐transfected MSCs were intradermally injected to CD18−/− wounds at 2.5 × 105 per wound. The transplantation of untransfected MSCs or control siRNA‐transfected MSCs at same numbers served as controls. Wound tissues were harvested at days 2, 5, and 7 post‐wounding. Genomic DNA was isolated from wound tissues, and the amount of human β‐actin DNA was quantified by qPCR normalized on mouse β‐actin. Mean ± SD, n = 3 wounds per group, not significant by one‐way ANOVA with Tukey's test.
  • B
    Quantification of immunofluorescence of active caspase 3 (C) and Ki67 (D). Mean ± SD, n = 3 wounds per group, not significant by one‐way ANOVA with Tukey's test.
  • C, D
    Representative immunofluorescence images of active caspase 3 (C) or Ki67 (D) (red) together with human β2M (green). Nuclei were counterstained with DAPI. Scale bars: 50 μm.