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. 2020 Feb 25;21(4):e49076. doi: 10.15252/embr.201949076

Figure EV1. RAP1 depletion does not affect telomere length.

Figure EV1

  1. RAP1 expression of dividing MRC‐5 cells (left panel; PD 26 and PD 66) and senescent cells (right panel; PD72 + 4 weeks). RAP1 depletion (or ectopic expression—right panel) was carried out for 10 days.
  2. Telomere restriction fragment of young (PD 26) and senescent cells (PD 72 + 4 weeks) upon 10‐day shRAP1 incubation.
  3. Single telomere length analysis (STELA) of MRC‐5 senescent cells transduced with either a control vector or an shRAP1‐expressing vector. STELA was performed at the Xp/Yp telomere, and the mean telomere length ± SD, after subtracting 406 bp of flanking DNA, is shown at the bottom of the blot.