. 2015 Oct 3;85:254–272. doi: 10.1016/j.envint.2015.09.018

Table 3.

Comparative metric and performance of three NG sequencers.

	Platforms
	Roche (454)	Illumina	SOLiD
Sequencing chemistry	Pyrosequencing	Polymerase based sequencing-by-synthesis	Ligation based sequencing
Amplification approach	Emulsion PCR	Bridge amplification	Emulsion PCR
Paired ends/Separation	Yes/3 kb	Yes/200 bp	Yes/3 kb
Mb/run	100 mb	1300 mb	3000 mb
Time/run (paired ends)	7 h	4 days	5 days
Read length	250 bp	32–40 bp	35 bp.