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. 2020 Jan;75:100666. doi: 10.1016/j.jbior.2019.100666

Fig. 1.

Fig. 1

Domain structures of the three components of LUBAC. The LUBAC is formed from three subunits, HOIL-1, HOIP and Sharpin. HOIL-1 and HOIP are both members of the Really Interesting New Gene (RING)-Between-RING (RBR) subfamily of E3 ubiquitin ligases, with catalytic cysteines located at C458 (mouse HOIL-1) and C879 (mouse HOIP) in the C-terminal RING2 domain. The Ubiquitin Associated (UBA) domain of HOIP binds simultaneously to the Ubiquitin Like (UBL) domains of HOIL-1 and Sharpin. HOIP also contains a PUB (peptide:N-glycanase/UBA- or UBX-containing protein) domain, which interacts with the deubiquitylases Otulin and CYLD (cylindromatosis), the latter via the adaptor protein SPATA2 (spermatogenesis-associated protein 2).