Fig. 3.

A hydroxylamine-sensitive modification of HOIL-1. (A) Immunoblots of extracts of bone-marrow-derived macrophages (BMDM) (20 μg protein) derived from wild-type (WT) and HOIL-1[C458S] knock-in mice. (B) LUBAC was immunoprecipitated with anti-HOIP from the extracts of WT macrophages, then treated with the protein phosphatase from bacteriophage lambda (λPPase), USP2 or hydroxylamine (NH₂OH), followed by immunoblotting with the antibodies indicated. Adapted from results shown in Kelsall et al. (2019).