Figure 7.

IFITM1 Has Anti-Viral Properties and Partly Restricts MV Infection

(A) Cell viability of 5H cells and IFITM1-overexpressing 5H cells was assessed by trypan blue exclusion at 24 and 48 hpi with MV-NSe (MOI of 1.0). Data are expressed as a percentage of cell killing by MV relative to mock-infected control cells (n = 3). (B) MV-N gene expression measured by qRT-PCR at 24 hpi. Results shown are relative to GAPDH and normalized to mock-infected control cells (n = 3). (C) Representative fluorescence microscopy images of MV-GFP-infected non-transduced and 5H-IFITM1 cells. Scale bars, 300 μm. (D) IFITM1 mRNA expression was measured by qRT-PCR at 24 hpi. Results shown are relative to GAPDH and normalized to mock-infected control cells (n = 3). All data are expressed as mean ± SEM (unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001). MV, measles virus; UI, uninfected; NS, not significant.