Figure 5.

Chemotherapy in HCC Is More Efficient Together with Downregulation of the Arg/N-degron Pathway

(A) Liver/body mass ratio analysis of mice bearing HCC treated for 4 weeks with LNPs at 1 mg/kg, with or without doxorubicin treatment; n = 10–18 mice per group. Results show mean ± SEM. (B) Weight curve of animals treated with LNPs, measured before each injection and averaged for the group. Measures were compared as a percentage of change from the first injection day. (C) Liver sections stained for the expression of Ki67 as a measure of cell proliferation; n = 4 mice per group. (D) Analysis of cell death by TUNEL assay in liver sections. Red nuclei represent TUNEL-positive cells. Scale bars, 100 μm; n = 2–3 mice per group. Results show mean ± SD. p values were determined by Mann-Whitney tests (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).