Figure 4.

Combined Intratumoral Electrotransfer of DNA-Based Checkpoint Inhibitors

MC38-bearing C57BL/6J mice received three intratumoral (i.t.) electroporations (indicated by arrows) of 60 μg p(aCTLA-4) or p(CAG-maPD-1) in 25 μL D-PBS, or the combination of p(aCTLA-4) and p(CAG-maPD-1) or an equimolar amount of pNull in 50 μL D-PBS. One group received no treatment. (A) Tumor growth and number of complete responders (CR). Tumor volumes were compared with one-way ANOVA on day 18 after tumor cell injection, when the first mice had to be sacrificed (**p < 0.01, ****p < 0.0001). (B) Anti-CTLA-4 (black lines) and anti-PD-1 (gray lines) mAb plasma concentrations in the single-treatment groups (solid lines) and combination-treatment group (dashed lines). All data are represented as mean + SEM (n = 10 mice per group). For Kaplan-Meier survival curves, see Figure S6.