Table 1.
Characteristics of Anti-CTLA-4 and Anti-PD-1 mAb-Encoding pDNA Constructs
| Promoter | Variable Regions | Isotype | Plasmid Design | Plasmid Backbone | |
|---|---|---|---|---|---|
| p(aCTLA-4-HC+LC) | CAG (two in tandem) | murine 9D9 | IgG2ab | single pDNA | pNull |
| p(aCTLA-4) = p(aCTLA-4-HC) + p(aCTLA-4-LC) | CAG | murine 9D9 | IgG2ab | equimolar mixture of HC-pDNA and LC-pDNA | pNull |
| p(CAG-caPD-1-HC2a+LC) | CAG (two in tandem) | rat RMP1-14 | IgG2aa | single pDNA | pNull |
| p(CMV-caPD-1-HC1) + p(CMV-caPD-1-LC) | CMV | rat RMP1-14 | IgG1 | equimolar mixture of HC-pDNA and LC-pDNA | pControl |
| p(CMV-maPD-1) = p(CMV-maPD-1-HC1) + p(CMV-maPD-1-LC) | CMV | murinized RMP1-14 | IgG1 | equimolar mixture of HC-pDNA and LC-pDNA | pControl |
| p(CAG-maPD-1) = p(CAG-maPD-1-HC1) + p(CAG-maPD-1-LC) | CAG | murinized RMP1-14 | IgG1 | equimolar mixture of HC-pDNA and LC-pDNA | pNull |
HC, heavy chain; LC, light chain; pDNA, plasmid DNA.