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. 2020 Feb 7;28(4):1200–1213. doi: 10.1016/j.ymthe.2020.02.003

Figure 1.

Figure 1

Characterization of HucMSC-exos

(A) Western blot analysis of exosome-related markers Alix, Tsg101, and CD9. Plasma protein Gm130 was used as a negative control. Both lanes were loaded with 20 μg of proteins as measured by Qubit 3.0. WCL, whole-cell lysate; Exo, exosome. (B) Representative image of HucMSC-exos observed by transmission electron microscope (TEM). Scale bar, 100 μm. (C) Nanoparticle tracking analysis of size distribution and concentration of HucMSC-exos. The mean protein concentration and mean particle concentration of the HucMSC-exos were 0.79 mg/mL and 2.7 × 1010 particles/mL, respectively.