Figure 2.

Uptake of HucMSC-exos by Cultured Newborn Ovaries

Newborn ovaries (P2.5) were incubated with PKH67-labeled exos for 0, 3, 6, 12, and 24 h. (A) Gradual accumulation of HucMSC-exos in primordial follicles. Ovaries were collected for frozen sections and nuclei were stained with Hoechst 33342. Green indicates exos; blue shows Hoechst 33342. For the control (Ctrl), ovaries were incubated with PBS for 24 h. (B) Fluorescence intensity analysis of labeled HucMSC-exos. n = 4/group. (C) Internalization of HucMSC-exos after ovaries were cultured for 24 h in the presence (left panel) or absence (right panel) of 5,000 IU of heparin (Hep). (D) Fluorescence intensity analysis of internalized HucMSC-exos. n = 4/group. Insets represent magnification of representative primordial follicles marked by white arrows. Dotted circles indicate primordial follicles. Data are shown as mean ± SEM. ***p < 0.001. Scale bars, 20 μm.