Figure 7.

Stimulation of Follicular Development in Old Mice by HucMSC-exos

(A) Timeline of HucMSC-exos treatment. Mice at 10 months of age were intrabursally injected with 20 μL of HucMSC-exos or the same volume of PBS. For some mice, one lateral ovary was given HucMSC-exos and the other one received PBS. Ovaries were collected for morphology 3 weeks later. Some mice were injected with HucMSC-exos or PBS bilaterally for a 4-month fertility test. (B) Distribution of follicles at different developmental stages in ovaries treated with PBS (Ctrl) or HucMSC-exos (Exo). Primo, primordial follicle; Prima, primary follicle; Sec, secondary follicle; EA, early antral follicle; LA, late antral follicle; CL, corpus luteum. n = 5/group. (C) Number of mice that gave birth to pups during the test. (D) Comparison of the cumulative numbers of pups per female in the control group (blue) and HucMSC-exo-treated (red) group. Data are shown as mean ± SEM. *p < 0.05.