Skip to main content
. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Feb 19;525(2):259–264. doi: 10.1016/j.bbrc.2020.02.059

Figure 4.

Figure 4.

Binding of UAP56-NTE and RNA to NP* is mutually exclusive. (A-B) EMSA assay of Alexa 488-labeled U15 RNA binding to NP* (A) and NP*-R174D/R175D (B). (C) U15 RNA displaced FITC-UAP56-NTE1-19 from the NP*•UAP56-NTE1-19 complex in a concentration dependent manner. All experiments (A-C) were repeated three times independently. (D) Working model of how UAP56 facilitates NP-mediated influenza genome packaging. Our results suggest that UAP56 initially acts on RNA-free NP through interactions with both NTE and the core of UAP56. Such configurations may prime NP for RNA interaction. Upon NP deposition on viral RNA, UAP56 is displaced due to the mutually exclusive binding of UAP56-NTE and RNA to NP.