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. 2020 Jan 21;28(4):1214–1228. doi: 10.1016/j.ymthe.2020.01.020

Figure 2.

Figure 2

MSC Expression of CX3CR1 and IL-25 following LV Infection

(A) The expression level of CX3CR1 was examined in MSCs after infection with a LV encoding both CX3CR1 and IL-25 at different time points. (B) The expression level of CX3CR1 was examined in MSCs after treatment with different types of LVs at 96 h post-infection. (C) Representative flow cytometry images of CX3CR1 expression levels in MSCs administered the indicated treatments. (D) The quantification results of CX3CR1 positive cells in MSCs. (E) The protein levels of IL-25 in MSCs treated with CX3CR1&IL-25-LV at different time points were assayed by ELISA. (F) The protein levels of IL-25 in MSCs 96 h after different types of LV infection were assayed by ELISA. (G) Immunofluorescence staining for CX3CR1 and IL-25 was performed with empty-LV-infected MSCs and CX3CR1&IL-25-LV-MSCs (96 h post-infection; red indicates CX3CR1, green indicates IL-25, and blue indicates DAPI nuclear staining). Scale bars, 10 μm. For cell experiments, five samples were analyzed per condition, and the experiments were performed in triplicate. The western blotting data shown are representative of three individual analyses. Values are expressed as the mean ± SEM. ***p < 0.001; n.d., not detected.