Figure 3.

Phenotypic Characterization and Immunogenicity Analysis of following LV Infection

(A) The morphologies of MSCs treated with different types of LVs at passage 2. Scale bars, 20 μm. For cell experiments, five samples were analyzed per condition, and the experiments were performed in triplicate. (B) Flow cytometry analysis of immune cell markers (CD11b and CD45) and stem cell markers (CD29 and CD90) in MSCs given different treatments. (C) Cell proliferation rates of MSCs given the indicated treatments, as determined using a CCK-8 assay. NS, no significant change, compared between MSCs and CX3CR1&IL-25-LV-MSCs. (D) Flow cytometry analysis of markers related to immunogenicity (CD80, CD86, CD40, MHC-I, and MHC-II) in MSCs with LV infection. (E) Lymphocytes from mice with DSS-induced colitis were co-cultured with LV-infected MSCs for 72 h, and then the proliferation rates of lymphocytes were determined by a CCK-8 assay. Stimulation index (SI): for co-culture groups, SI = (sample OD − basal OD)/negative control OD; for lymphocytes and lymphocytes + Con A groups, SI = sample OD/negative control OD. Values are expressed as the mean ± SEM. ***p < 0.001. NS, no significant change.