Figure 5. Immunohistochemistry of Canine and Human Gliomas.
(A) Representative hematoxylin & eosin and immunohistochemistry staining of human adult (n = 11), canine (n = 11), and human pediatric gliomas (n = 5) using antibodies against T cells (CD3), macrophage/microglia (IBA1), M2 polarized innate immune cells (CD163), monocytes (CD14), and B cells (CD79A). Scale bars, 50 μm.
(B) Violin plots represent the density of percentage positivity by field (y axis) for each of five antibodies described in (A). The points are the mean value of percentage positivity per patient within each of three cohorts, i.e., human adult (n = 11), canine (n = 11), and human pediatric gliomas (n = 5). Patients were grouped into high- versus low-grade gliomas in the absence of available molecular subtype data.
See also Figure S5.