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. 2020 Feb 5;28(4):1119–1132. doi: 10.1016/j.ymthe.2020.02.002

Figure 4.

Figure 4

Regulation of Gene Expression via Trans-Elemental 3′ UTRs

(A–C) The purified mouse neocardiomyocytes were pre-cultured with AAV-GFP or AAV-Mc-U. Real-time PCR (A) and western blot (B) analyses were performed for Aggf1 and Mcl1 expression after treatment. (C) The secretory Aggf1 levels were detected by ELISA assays. n = 6. *p < 0.05 versus AAV-GFP. (D and E) The purified mouse neocardiomyocytes were pre-cultured with AAV-GFP or AAV-Ag-U. Real-time PCR analyses for Aggf1 and Mcl1 mRNA levels (D) and ELISA assays for secretory Aggf1 levels (E) were performed. n = 6. *p < 0.05 versus AAV-GFP. (F) The luciferase activities of pLuc-AG-U and pLuc-MC-U were measured in the deficiency of MCL1 or AGGF1 in HEK293 cells. Statistical analysis was carried out with a Student’s t test. n = 6. *p < 0.05 versus siScr.