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. 2020 Mar 30;13(3):dmm042515. doi: 10.1242/dmm.042515

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Arx^(GCG)10+7 expansion mutants do not exhibit an astrocytic response at P7. (A-H) Representative images from parasagittal slices showing GFAP immunohistochemistry (red) and DAPI nuclei (blue) from WT (A-D) and Arx^(GCG)10+7 (E-H) littermates. Images show each region quantified: RSA (A,E), white matter (WM; B,F), CA1 of the hippocampus (CA1; C,G) and dentate gyrus of the hippocampus (DG; D,H). (I-L) Mean fluorescence quantified from each region specified and graphs display average values for each genotype in the RSA (P=0.4686, unpaired Student's t-test) (I), WM (P=0.6277, unpaired Student's t-test) (J), CA1 (P=0.7385, unpaired Student's t-test) (K) or DG (P=0.2139, unpaired Student's t-test) (L). N=8 WT, N=8 mutant. Scale bars: 100 µm. Means±s.e.m. are displayed, with N values displayed on graphs.