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. 2020 Mar 30;147(6):dev186213. doi: 10.1242/dev.186213

Fig. 3.

Fig. 3.

Stage-specific expression of islet factors during development. (A-C) Immunostaining of INS (green) and GCG (white) paired with SST (red) (A), E-cadherin (ECad; red) (B) and chromogranin-A (CHGA; red) (C) in the early fetal, neonatal and adult pig pancreas. (D-F) Immunostaining of the TFs PDX1 (D), NKX6.1 (E) and MAFB (F) in the early fetal, neonatal and adult pig pancreas. (G) Immunostaining of SIX3 with INS and GCG in adult pig pancreas. Insets shows magnification of dashed yellow boxed area. (H,I) qRT-PCR measures of relative mRNA levels encoding SIX3 (H) and SIX2 (I) in isolated neonatal and adult pig islets (n=2, t-test; error bars show s.d.). ΔCT values for neonatal and adult SIX3 expression were 5.1 and 2.0, respectively (compared with β-actin). ΔCT values for neonatal and adult SIX2 (compared with β-actin) were 7.2 and 2.6, respectively. Scale bars: 50 µm.