Figure 2.

TUG1 directly interacted with miR-27a. IntaRNA (http://rna.informatik.uni-freiburg.de/IntaRNA/Input.jsp) was used to predict the potential interaction between TUG1 and miR-27a (a). Dual luciferase reporter assay was performed by transfecting AC16 cells with TUG1 vector + NC miRNA (NC group) or TUG1 vector + miR-27a mimic (miR-27a group) (b). Data were compared by unpaired t-test. Experiments were repeated three times and mean values are shown; error bars represent standard deviations. *P < 0.05. TUG1, taurine-upregulated gene 1; AC16, human cardiomyocyte cell line; NC, negative control.