Figure 3.

TUG1 overexpression led to decreased TNFA mRNA expression. The interaction between TUG1 and miR-27a was further explored by transfecting TUG1 expression vector or miR-27a mimic into AC16 cells. Overexpression of TUG1 and miR-27a was confirmed by qPCR at 24 hours post-transfection (a). The effect of TUG1 or miR-27a overexpression on the expression of each other was also analyzed by qPCR at 24 hours post-transfection (b). Effects of TUG1 and miR-27a on the expression of TNFA in AC16 cells were analyzed by qPCR and western blot at the mRNA (c) and protein (d) levels, respectively. Experiments were repeated three times and mean values are shown; error bars represent standard deviations. *P < 0.05. TNFA, tumor necrosis factor-α gene; TUG1, taurine-upregulated gene 1; AC16, human cardiomyocyte cell line; qPCR, real-time quantitative PCR.