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. 2020 Mar 6;68(4):279–294. doi: 10.1369/0022155420911930

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Figure 2. — Histochemistry of Brunner’s glands and villi of control and HFD mice. (A) Brunner’s glands in the control specimen show smaller lumina (arrows) and secretion (double headed arrow) is intensely stained by PAS for carbohydrates and distributed rather uniformly. (B) In respect to control, Brunner’s glands in the HFD condition present larger lumina (arrows) and the PAS-positivity of the secretion is weaker (double headed arrow), concentrated in the subapical area of cells. (C–F) Brunner’s glands in both control (C, E) and HFD (D, F) are negative to AB pH 2.5 (C, D) and HID (E, F) stains for acidic residuals. (G, H) Villi present goblet cells (arrows) and glycocalyx (double headed arrows) positive to PAS stain without difference of intensity between control (G) and HFD (H). (I, J) Villi present goblet cells (arrows) and glycocalyx (double headed arrows) positive to AB pH 2.5 stain for acidic residuals with no apparent difference of intensity between control (I) and HFD (J). (K, L) Villi present goblet cells (arrows) and glycocalyx (double headed arrows) positive to HID stain for sulfated glycans that is more intense in control (K) than in HFD (L). (A, B, G, H) PAS-hematoxylin. (C, D, I, J) Alcian blue pH 2.5-hematoxylin. (E, F, K, L) HID-hematoxylin. Scale bar=50 µm. Abbreviations: HFD, high-fat diet; PAS, periodic acid-Schiff; HID, high iron diamine; AB, alcian blue.