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Inter-day and intra-day precision of the HPTLC method (n = 6) for protopine (mean ± SD).
| Amount (ng/spot) | Inter-day precision |
Intra-day precision |
||
|---|---|---|---|---|
| Mean peak area ± SD | %RSD | Mean peak area ± SD | %RSD | |
| Protopine | ||||
| 2000 | 2476.08 ± 47.61 | 1.923175 | 2461.35 ± 51.07 | 2.07 |
| 3000 | 3474.35 ± 17.14 | 0.49335 | 3459.60 ± 26.23 | 0.75 |
| 4000 | 4876.58 ± 70.17 | 1.438938 | 4887.93 ± 52.84 | 1.08 |
RSD: Regressed Standard Deviation.
