Skip to main content
. 2020 Jan 15;28(4):1154–1166. doi: 10.1016/j.ymthe.2020.01.013

Figure 1.

Figure 1

miR-206 Was Upregulated in the Brainstem of SMA Mice Parallel to MN Loss in the Facial Nuclei in the Early Phase of the Disease

(A and B) Real-time PCR of miR-206 for the evaluation of its expression levels in the brainstem (A) and in the spinal cord (B) of WT (white columns) and SMA (black) mice, five (P5) and 12 (P12) days after birth showing miR-206 upregulation at P5 in the brainstem and at P12 in the spinal cord. (C) Nissl-stained sections (20 μm) of the brainstem of WT (a and b) and SMA (c and d) mice at P5 (a and c) and P12 (b and d) showing MN reduction in the facial nuclei (ellipse area) of SMA mice. Scale bars 200 μm. (D) Quantification of MN number normalized for photographic field in the facial nuclei of WT and SMA mice at P5 and P12. *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001 by one-way ANOVA analysis followed by Bonferroni test. Each column represents the mean ± SEM (n = 3/6).