Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 5;8:42. doi: 10.1186/s40478-020-00916-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Identification of candidate ANXA2-regulated genes that promote angiogenesis and cell invasion. a Microarray analysis was performed to identify ANXA2-regulated genes that promote the angiogenesis–invasion phenotype. See text for the definitions of Angiogenesis-1 and Angiogenesis-2 genes. b Venn diagram of Angiogenesis-1, Angiogenesis-2, and shared genes identified from the analysis in (a). c Anatomical expression pattern of ANXA2 mRNA in the brain from the Ivy Glioblastoma Atlas Project dataset (n = 270). LE, leading edge; IT, infiltrating tumor; CT, cellular tumor; PZ, perinecrotic zone; PAN, pseudopalisading cells around necrosis; HBV, hyperplastic blood vessels; MVP, microvascular proliferation. Data are shown as the mean ± SEM. ***P < 0.001, NS not significant by one-way ANOVA with Bonferroni’s post hoc test. d Hierarchical clustering of genes related to angiogenesis and invasion ordered by anatomical location (Ivy GAP dataset, n = 270). e Hierarchical clustering of genes related to angiogenesis and invasion ordered by GBM molecular subtypes GBM (TCGA dataset, n = 143). f–h Pearson’s correlation tests of ANXA2 and OSMR mRNA expression in GBM datasets from TCGA (F, n = 147) and REMBRANDT (g, n = 214) and from GBM samples from Okayama University (h, n = 40). i Western blot analysis of ANXA2, OSMR, and β-actin in established human GBM cell lines and patient-derived GBM cells. j Pearson’s correlation test of ANXA2 and OSMR protein expression from the data shown in (i)