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. 2020 Apr 6;11:149. doi: 10.1186/s13287-020-01660-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Candidate subpopulations with different functional characteristics. a UMAP visualizing the results of cell clustering. b Bean plots showing expression of several representative DEGs among the six subpopulations. c Pathways significantly enriched for the genes differentially expressed in one subpopulation compared to others. d GO-slim biological process enriched for the genes differentially expressed in one subpopulation compared to others. For c and d, only the top 10 terms with lowest FDR (FDR ≤ 0.05) were present. e Boxplots showing expression of classic MSC marker genes in subpopulations. f Example of candidate markers showing different expression pattern among the five subpopulations (C0–C4). C0, red; C1, olive; C2, yellow; C3, green; C4, blue; C5, purple. IMCC, inflammation mediated by chemokine and cytokine; DNPD, de novo pyrimidine deoxyribonucleotide