Table 1.
Antibody and serum information
| Primary antibodies | ||||
| Antibody target | Company | Species | Dilution | Serum |
|---|---|---|---|---|
| Cholecystokinin | Frontier Institute | Rabbit | 1:750 | NHS |
| Calretinin | Swant | Goat | 1:1000 | NDS |
| Somatostatin | Santa Cruz Biotechnology | Rabbit | 1:200 | NHS |
| GAD67 | Millipore | Mouse | 1:2000 | NHS |
| APP695 | Thermo Fisher | Mouse | 1:1000 for both IF and IP | NHS |
| CD68 | Bio-Rad | Goat | 1:500; 1:3000 for IP | NGS |
| GFAP | Agilent (Dako) | Rabbit | 1:500; 1:2000 for IP | NGS |
| Secondary antibodies | ||||
| Immunofluorescence | ||||
| Texas Red | Thermo Scientific | Rabbit | 1:500 | |
| FITC | Sigma-Aldrich | Mouse | 1:875 | |
| Alexa 488 | Abcam | Rabbit | 1:1000 | |
| Alexa 568 | Molecular Probes | Goat | 1:500 | |
| DAPI | Sigma-Aldrich | Multiple | 1:1000 | |
| Immunoperoxidase | ||||
| Biotinylated | Vector Laboratories | Mouse, goat, rabbit | 1:500 | |
IF, immunofluorescence; IP, immunoperoxidase; NGS, normal goat serum.
The “Serum” column refers to the serum used for the 1-h incubation step before placement in primary antibodies and the serum used in conjunction with the secondary antibody solution.