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PANC1 and MiaPaca2 cells were treated with vehicle control or with neratinib (50 nM) for 4h and 8h. At each time point the cells were fixed in place and immunostaining performed to detect the phosphorylation and expression of the indicated proteins (n = 3 +/− SD) * p < 0.05 less than vehicle control; # p < 0.05 greater than vehicle control.
