Table 1.
Features of TB antigens identified as putative biomarkers by serological screening of proteome microarrays.
| Locus tag-Ab type | ATB/LTBI+HC | P value | Gene symbol | Gene product description |
|---|---|---|---|---|
| Cell wall and cell processes (10, 17.24%) | ||||
| MT3503-IgM | 1.024 | 0.0031 | Rv3395A | Probable membrane protein |
| Rv0435c-IgM | 1.040 | 0.0273 | Rv0435c | ATPase |
| Rv1473-IgM | 1.034 | 0.0421 | Rv1473 | Macrolide ABC transporter ATP-binding protein |
| Rv1821-IgG | 1.015 | 0.0097 | secA2 | Accessory Sec system translocase SecA2 |
| Rv1860-IgG | 1.144 | 0 | apa | Alanine and proline-rich secreted protein Apa |
| Rv1860-IgM | 1.110 | 0 | apa | Alanine and proline-rich secreted protein Apa |
| Rv2450c-IgM | 1.051 | 0.0284 | rpfE | Resuscitation-promoting factor RpfE |
| Rv2462c-IgG | 1.019 | 0.0024 | tig | Trigger factor |
| Rv2688c-IgM | 1.056 | 0.0081 | Rv2688c | Antibiotic ABC transporter ATP-binding protein |
| Rv3810-IgG | 1.045 | 0.0064 | pirG | Cell surface protein |
| Conserved hypothetical proteins (10, 17.24%) | ||||
| MT1547-IgG | 1.033 | 0.0075 | MT1547 | Hypothetical protein |
| Rv0049-IgG | 1.014 | 0.037 | Rv0049 | Hypothetical protein |
| Rv0049-IgM | 1.046 | 0 | Rv0049 | Hypothetical protein |
| Rv1100-IgG | 1.040 | 0.038 | Rv1100 | Hypothetical protein |
| Rv1100-IgM | 1.092 | 0 | Rv1100 | Hypothetical protein |
| Rv1597-IgM | 1.066 | 0.0012 | Rv1597 | Hypothetical protein |
| Rv2342-IgG | 1.029 | 0.0406 | Rv2342 | Hypothetical protein |
| Rv3472-IgG | 1.026 | 0.0241 | Rv3472 | Hypothetical protein |
| Rv3542c-IgG | 1.027 | 0.0004 | Rv3542c | Hypothetical protein |
| Rv3542c-IgM | 1.027 | 0.0292 | Rv3542c | Hypothetical protein |
| Information pathways (2, 3.45%) | ||||
| Rv0684-IgM | 1.005 | 0.0115 | fusA1 | Elongation factor G |
| Rv0944-IgM | 1.053 | 0.0218 | Rv0944 | Formamidopyrimidine-DNA glycosylase |
| Intermediary metabolism and respiration (17, 29.31%) | ||||
| MT1560.1-IgG | 1.040 | 0.0368 | MT1560.1 | Hypothetical protein |
| Rv0363c-IgG | 1.025 | 0.0002 | fba | Fructose-bisphosphate aldolase |
| Rv0408-IgM | 1.025 | 0.0072 | Pta | Phosphate acetyltransferase |
| Rv0457c-IgG | 1.019 | 0.0342 | Rv0457c | Peptidase |
| Rv1001-IgM | 1.049 | 0.0016 | arcA | Arginine deiminase |
| Rv1320c-IgG | 1.027 | 0.0204 | Rv1320c | Adenylate cyclase |
| Rv1876-IgG | 1.030 | 0.019 | bfrA | Bacterioferritin BfrA |
| Rv1876-IgM | 1.083 | 0 | bfrA | Bacterioferritin BfrA |
| Rv2179c-IgG | 1.007 | 0.0031 | Rv2179c | 3′-5′ exoribonuclease |
| Rv2368c-IgG | 1.028 | 0.0064 | phoH1 | Phosphate starvation-inducible protein PhoH |
| Rv2368c-IgM | 1.022 | 0.0033 | phoH1 | Phosphate starvation-inducible protein PhoH |
| Rv2511-IgG | 1.036 | 0.0007 | orn | Oligoribonuclease |
| Rv3248c-IgG | 1.046 | 0.0001 | sahH | Adenosylhomocysteinase |
| Rv3248c-IgM | 1.024 | 0.0092 | sahH | Adenosylhomocysteinase |
| Rv3712-IgG | 1.018 | 0.0083 | Rv3712 | Ligase |
| Rv3838c-IgG | 1.019 | 0.0414 | pheA | Prephnate dehydratase |
| Rv3841-IgG | 1.022 | 0.0121 | bfrB | Bacterioferritin BfrB |
| Lipid metabolism (5, 8.62%) | ||||
| Rv0270-IgM | 1.017 | 0.0096 | fadD2 | Fatty-acid-CoA ligase |
| Rv1350-IgG | 1.019 | 0.0168 | fabG2 | 3-oxoacyl-ACP reductase |
| Rv3480c-IgM | 1.049 | 0.0028 | Rv3480c | Diacyglycerol O-acyltransferase |
| Rv3506-IgG | 1.038 | 0.0001 | fadD17 | Long-chain-fatty-acid-CoA ligase FadD17 |
| Rv3506-IgM | 1.047 | 0.0436 | fadD17 | Long-chain-fatty-acid-CoA ligase FadD17 |
| PE/PPE (3, 5.17%) | ||||
| Rv0280-IgM | 1.012 | 0.0187 | PPE3 | PPE family protein PPE3 |
| Rv2352c-IgG | 1.026 | 0.012 | PPE38 | PPE family protein PPE38 |
| Rv2352c-IgM | 1.060 | 0.0002 | PPE38 | PPE family protein PPE38 |
| Regulatory proteins (3, 5.17%) | ||||
| Rv0494-IgG | 1.002 | 0.016 | Rv0494 | HTH-type transcriptional regulator |
| Rv0494-IgM | 1.035 | 0.0033 | Rv0494 | HTH-type transcriptional regulator |
| Rv0576-IgG | 1.019 | 0.039 | Rv0576 | Transcriptional regulator |
| Virulence, detoxification and adaptation (8, 13.79%) | ||||
| Rv0174-IgM | 1.027 | 0.0216 | mce1F | MCE-family protein Mce1F |
| Rv0350-IgG | 1.047 | 0.0462 | dnaK | Chaperone protein DnaK |
| Rv0350-IgM | 1.083 | 0.014 | dnaK | Chaperone protein DnaK |
| Rv0351-IgM | 0.995 | 0.048 | grpE | Stress response protein GrpE |
| Rv0440-IgG | 1.090 | 0.0014 | groEL2 | Molecular chaperone GroEL |
| Rv2031c-IgG | 1.023 | 0.0045 | hspX | Alpha-crystallin |
| Rv2031c-IgM | 1.029 | 0.0027 | hspX | Alpha-crystallin |
| Rv3418c-IgG | 1.019 | 0.0466 | groES | Ahaperonin GroES |
Ab, antibody; ABC: ATP-binding cassette; ACP: acyl-carrier protein; ATB, active TB; ATP: adenosine triphosphate; Co-A: coenzyme A; HC, healthy control; HTH, helix-turn-helix; IgG, immunoglobulin G; IgM, immunoglobulin M; LTBI: latent TB infection; MCE, mammalian cell entry; PE: Pro-Glu; PPE: Pro-Pro-Glu; and TB, tuberculosis.