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. 2020 Mar 31;48(3):0300060520904847. doi: 10.1177/0300060520904847

Figure 2.

Figure 2.

Restoration of miR-34c significantly enhanced NSCLC cell chemosensitivity. (a) The indicated cells were transfected with miR-34c mimics or miR-34c ASO. At 72 hours after transfection, the expression of miR-34c was measured by qRT-PCR. (b) The indicated cells were treated with the indicated drugs and miR-34c ASO for 24 hours, and the cell viability was then analyzed by the CCK-8 assay. (c) The indicated cells were treated with the indicated drugs and miR-34c mimics for 24 hours, and the cell viability was then analyzed by the CCK-8 assay. (d) The indicated cells were treated with doxorubicin, cisplatin, or paclitaxel for 24 hours, and apoptosis was then analyzed by measuring the percentage of annexin V-positive cells using flow cytometry. (e) Cleaved caspase 3 was analyzed by western blot. miR, microRNA; NSCLC, non-small cell lung cancer; ASO, antisense oligonucleotides; qRT-PCR, quantitative real-time polymerase chain reaction, CCK-8, Cell Counting Kit-8. *p < 0.05, **p < 0.01.