Hypothesis: bipolar disorder is an Epstein–Barr virus‐driven chronic autoimmune disease – implications for immunotherapy

Abstract

Bipolar disorder (BD) is a chronic disease characterised by episodes of major depression and episodes of mania or hypomania, with a worldwide prevalence of 2.4%. The cause of BD is unknown. Here, I propose the hypothesis that BD is a chronic autoimmune disease caused by Epstein–Barr virus (EBV) infection of autoreactive B cells. It is postulated that EBV‐infected autoreactive B cells accumulate in the brain where they provide costimulatory survival signals to autoreactive T cells and differentiate into plasma cells producing pathogenic autoantibodies targeting brain components such as the N‐methyl‐D‐aspartate receptor. It is also proposed that the accumulation of EBV‐infected autoreactive B cells in the brain is a consequence of a genetically determined defect in the ability of CD8⁺ T cells to control EBV infection. The theory is supported by studies indicating that autoimmunity, EBV infection and CD8⁺ T‐cell deficiency all have roles in the pathogenesis of BD. According to the hypothesis, BD should be able to be treated by EBV‐specific T‐cell therapy and to be prevented by vaccination against EBV in early childhood. Exposure to sunlight or appropriate artificial light should also be beneficial in BD by augmenting CD8⁺ T‐cell control of EBV infection.

This article proposes the novel hypothesis that bipolar disorder (BD) is a chronic autoimmune disease caused by Epstein–Barr virus (EBV) infection of autoreactive B cells, which accumulate in the brain where they facilitate an autoimmune attack on brain components such as the NMDA receptor. It is postulated that the accumulation of EBV‐infected autoreactive B cells in the brain is a consequence of a genetically determined defect in the ability of CD8⁺ T cells to control EBV infection. According to the hypothesis, BD should be able to be treated by EBV‐specific T‐cell therapy and to be prevented by vaccination against EBV early in life.

Introduction

Bipolar disorder (BD) is a chronic disease characterised by episodes of major depression and episodes of mania or hypomania, ¹ with a worldwide prevalence of 2.4% when subthreshold BD is included. ² The cause of BD is unknown. The concordance rate of BD in monozygotic twins is 40–70%, ³ indicating that genetic factors have a major role in the development of the disease. Given that this concordance rate is substantially less than 100%, environmental factors are also likely to make a significant contribution to the pathogenesis of the disease. There is some evidence that BD is a chronic autoimmune disease. I have previously hypothesised that all human chronic autoimmune diseases are caused by Epstein–Barr virus (EBV) infection of autoreactive B cells, which accumulate in the target organ where they provide costimulatory survival signals to autoreactive T cells and differentiate into plasma cells producing pathogenic autoantibodies. ⁴ Here, I hypothesise that BD is an EBV‐driven chronic autoimmune disease. The hypothesis gives rise to predictions that can be tested. A crucial prediction derived from the hypothesis is that BD should be able to be treated by EBV‐specific T‐cell therapy and to be prevented by vaccination against EBV early in life. Exposure to sunlight or appropriate artificial light should also be beneficial in BD by augmenting CD8⁺ T‐cell control of EBV infection.

The article will proceed through the following sections: (1) the pathophysiology of BD; (2) evidence that BD is an autoimmune disease; (3) introduction to EBV; (4) introduction to EBV‐infected autoreactive B‐cell hypothesis of autoimmunity; (5) evidence for a role of EBV in the pathogenesis of BD; (6) evidence for a role of CD8⁺ T‐cell deficiency in BD; (7) the benefit of sunlight; (8) the role of stress; (9) proposed hypothesis for the development of BD; (10) testing the hypothesis, including the implications for immunotherapy and phototherapy; and (11) conclusion.

Pathophysiology of BD

The pathophysiology of BD is complex and incompletely understood. Functional magnetic resonance imaging studies of emotional processing in BD have demonstrated underactivity of the ventrolateral prefrontal cortex and overactivity in the limbic regions, thalamus and basal ganglia. ⁵ Diffusion tensor imaging studies of the brain in BD have shown decreased fractional anisotropy, indicating decreased structural connectivity, in the corpus callosum. ⁶ , ⁷ Histological studies of the brain in BD have revealed a decrease in hippocampal N‐methyl‐D‐aspartate (NMDA) receptors with open ion channels, but no decrease in kainate or AMPA receptors. ⁸ Consistent with these findings, there is also a decreased expression of transcripts for the NR1 and NR2A subunits of the NMDA receptor and for SAP102, an NMDA receptor‐interacting protein, in the hippocampus in BD. ⁹ It is possible that the decrease in hippocampal NMDA receptors in BD is caused by the anti‐NMDA receptor immunoglobulin G (IgG) antibodies that have been detected in individuals with mania. ¹⁰

The beneficial effect of lithium in bipolar disorder has been attributed to its ability to inhibit glutamatergic neurotransmission mediated through NMDA receptors. ¹¹ , ¹² It has been proposed that in psychoses, such as bipolar disorder, hypofunctional NMDA receptors on inhibitory GABAergic hippocampal interneurons cause underactivity of the interneurons, which then release less GABA, which in turn disinhibits the pyramidal neurons in the hippocampus so that they release more glutamate from their projections to the midbrain and corpus striatum. ¹³ This explanation could account for the apparent paradox that lithium, an agent thought to block glutamatergic neurotransmission, ¹¹ is beneficial in a disease, BD, in which there is a decrease in hippocampal NMDA receptors. ⁸

Evidence that BD is an autoimmune disease

One important clue to the likelihood of BD being an autoimmune disease comes from the observations that, like multiple sclerosis (MS), which is a chronic autoimmune disease of the central nervous system (CNS), BD tends to present clinically in adolescence and early adulthood and that it initially has a relapsing–remitting course, which later becomes progressive and unremitting. ¹⁴ Intrathecal IgG synthesis, a hallmark of MS, occurs in 30% of patients with BD compared with 4% of controls, ¹⁵ as well as in healthy siblings of MS patients. ¹⁶ It has been hypothesised that intrathecal IgG synthesis with oligoclonal bands is caused by clonal expansion of EBV‐infected autoreactive B cells in the CNS. ⁴ Increased serum/plasma interleukin‐6 levels in BD ⁷ , ¹⁷ , ¹⁸ provide further evidence for a role of the immune system in the pathogenesis of BD.

Another indication that BD is an autoimmune disorder is the concurrence of BD with other autoimmune diseases. One study found that 28.3% of patients with BD had autoimmune thyroiditis, as determined by the presence of anti‐thyroperoxidase antibodies, compared with 13.5% of the general population. ¹⁹ In particular, MS is strongly associated with BD and major depressive disorder – Carta and colleagues found that 46.7% of patients with MS had a mood disorder, 26.3% had major depressive disorder, and 9.9% had bipolar spectrum disorder, compared with 5.2%, 4.6% and 0.25% of controls, respectively. ²⁰ Furthermore, patients with coeliac disease have substantially higher prevalences of major depressive disorder and BD than do controls. ²¹ Conversely, patients with BD have increased serum anti‐gliadin IgG antibodies. ²² There is also an increased frequency of BD in each of the following autoimmune diseases: pemphigus, ²³ systemic lupus erythematosus, ²⁴ , ²⁵ rheumatoid arthritis, ²⁵ autoimmune vasculitis, ²⁵ Sjögren's syndrome ²⁵ and Crohn's disease. ²⁵ Moreover, the female children of parents with BD have an increased prevalence of autoimmune thyroiditis. ²⁶ The first‐degree relatives of individuals with schizophrenia, a possible autoimmune disease, have not only an increased risk of developing schizophrenia but also an increased risk of developing BD. ²⁷ It is important to note that the above studies show that there is an immune dysregulation in a subset of patients with BD; they do not show that all patients with BD have immune dysregulation.

A key question is, if BD is an autoimmune disease, what is the target autoantigen? One obvious potential autoantigen is the NMDA receptor, which is decreased in the hippocampus in BD. ⁸ Support for the role of autoimmunity to the NMDA receptor in the pathogenesis of BD is provided by the observation that some patients presenting with mania have subsequently been diagnosed as having anti‐NMDA receptor encephalitis, ²⁸ , ²⁹ , ³⁰ which is an autoimmune disease of the CNS mediated by antibodies targeting the NR1 subunit of the NMDA receptor and leading to the internalisation of the receptor. ³¹ Importantly, patients with acute mania have been found to have increased serum IgG antibodies to the NR2 subunit of the NMDA receptor; these antibodies had declined substantially at follow‐up 6 months later. ¹⁰ It remains to be determined whether these antibodies directed against the NR2 subunit of the NMDA receptor are pathogenic. An autoimmune process in BD might also target brain components other than the NMDA receptor, such as molecules associated with the NMDA receptor and its signalling pathway.

Introduction to EBV

Epstein–Barr virus is a ubiquitous double‐stranded DNA γ‐herpesvirus infecting 90–95% of the adults in the world. EBV is the only human virus that infects, activates, clonally expands and persists latently in B lymphocytes for the duration of the infected individual's life. To achieve this, EBV exploits the normal B‐cell differentiation pathways. ³² In primary infection, EBV enters the tonsil from saliva and infects naïve B cells, forcing them out of the resting state so that they become activated proliferating B blasts. The B blasts then enter a germinal centre where they pass through a germinal centre reaction and differentiate into latently infected memory B cells that circulate in the blood ³² (Figure 1). On returning to the tonsil, latently infected memory B cells can differentiate into plasma cells, which initiates the lytic phase of infection. ³³ This culminates in the generation of virions. The newly formed virions infect epithelial cells in the tonsil where the virus reproduces rapidly and is continuously released into saliva to be transmitted to new hosts. ³⁴ The new virions also infect naïve B cells in the same host in order to complete the cycle required for EBV to persist as a lifelong infection. ³⁵ EBV deploys a series of different transcription programmes in order to pass through the discrete stages of its life cycle. ³² In order to activate the blast phase after infecting naïve B cells, EBV uses the latency III transcription programme to express all its latent proteins, namely Epstein–Barr nuclear antigen 1 (EBNA1), EBNA2, EBNA3A, EBNA3B, EBNA3C and EBNALP, and latent membrane protein 1 (LMP1), LMP2A and LMP2B. The infected blast enters a germinal centre where it turns off expression of EBNA2, EBNA3A, EBNA3B, EBNA3C and EBNALP and continues to express EBNA1, LMP1 and LMP2 (latency II transcription programme). During its passage through the germinal centre phase, the EBV‐infected B blast differentiates into a memory B cell. Latently infected memory B cells cannot be detected by EBV‐specific T cells or antibodies because they express no viral proteins except during homeostatic cell division, when they express only EBNA1 (latency I transcription programme), which is required to duplicate the EBV genome and transmit it to daughter cells. In order to generate new infectious virions, EBV infection becomes reactivated through the lytic transcription programme when latently infected memory B cells differentiate into plasma cells. ³³

Figure 1.

Normal sequence of events during infection of the tonsil by EBV. During primary infection, EBV enters the tonsil from the saliva and infects naïve B cells, driving them out of the resting state to become activated proliferating B blasts. The B blasts enter germinal centres where they proliferate intensely and differentiate into latently infected memory B cells, which then exit from the tonsil and circulate in the blood. The infected memory B cells do not express any viral proteins except during cell division when they express EBNA1. When latently infected memory B cells returning to the tonsil differentiate into plasma cells, the lytic phase of infection is initiated and free virus particles (virions) are produced. The virions infect tonsil epithelial cells where the virus replicates at a high rate and is shed into saliva for transmission to new hosts. Newly formed virions also infect additional naïve B cells in the same host, thereby completing the cycle necessary for the persistence of EBV in a lifelong infection. During primary infection, this cycle initially proceeds unchecked by the immune system. However, the infected host soon mounts an immune response against the virus. EBV‐specific cytotoxic CD8⁺ T cells kill infected cells expressing viral proteins, and anti‐EBV antibodies neutralise viral infectivity by binding to free virus. Red lines with perpendicular bars indicate inhibition. This model is based on the work published by Thorley‐Lawson and colleagues. ³² , ³³ , ³⁴ , ⁹¹ Modified from Pender ⁶² through a Creative Commons Licence.

In healthy individuals, EBV infection is rigorously controlled by EBV‐specific immune responses. In particular, cytotoxic CD8⁺ T cells play a major role in regulating EBV infection. By targeting EBV‐encoded latent and lytic proteins, EBV‐specific CD8⁺ T cells kill proliferating and lytically infected cells, respectively ³⁶ , ³⁷ (Figure 1).

Introduction to the EBV‐infected autoreactive B‐cell hypothesis of autoimmunity

Genes contributing to the pathogenesis of chronic autoimmune diseases consist of those causing a general susceptibility to autoimmunity and those predisposing to specific autoimmune diseases. One common manifestation of a general susceptibility to autoimmunity is an increased frequency of other autoimmune diseases in individuals with a particular autoimmune disease and in their blood relatives. ³⁸ , ³⁹ , ⁴⁰ , ⁴¹ It is likely that the general susceptibility to autoimmunity has a Mendelian‐dominant inheritance pattern. ³⁸ I have proposed that the general predisposition to autoimmunity is due to a genetic CD8⁺ T‐cell deficiency causing defective CD8⁺ T‐cell control of EBV infection. ⁴² The best‐characterised genes causing susceptibility to specific autoimmune diseases are HLA class II and less frequently HLA class I alleles. ⁴³ It is likely that the corresponding HLA molecules determine the autoantigens and therefore the target organs, which are recognised by autoreactive T cells activated by cross‐reacting foreign antigens or modified autoantigens.

The EBV‐infected autoreactive B‐cell hypothesis of autoimmunity, which was published in 2003, proposes that EBV infection of autoreactive B cells causes human chronic autoimmune diseases. ⁴ , ⁴² It postulates that a genetic defect in CD8⁺ T‐cell control of EBV infection allows the accumulation of EBV‐infected autoreactive B cells in the target organ. It also postulates that EBV‐infected autoreactive B cells in the target organ provide costimulatory survival signals to autoreactive T cells that would otherwise undergo activation‐induced apoptosis and die in the target organ. Furthermore, it is proposed that the now‐surviving autoreactive T cells provide T‐cell help to the EBV‐infected autoreactive B cells, which then differentiate into plasma cells producing pathogenic autoantibodies. The likelihood of EBV infecting autoreactive naïve B cells is considerable because at least 20% of human naïve B cells are autoreactive. ⁴⁴ Several predictions derived from the hypothesis have subsequently been substantiated, namely the accumulation of EBV‐infected B cells and plasma cells in the brain in MS ⁴⁵ , ⁴⁶ , ⁴⁷ , ⁴⁸ , ⁴⁹ ; EBV infection of autoreactive memory B cells in acute infectious mononucleosis ⁵⁰ ; EBV infection of autoreactive plasma cells in the joints in rheumatoid arthritis ⁵¹ and salivary glands in Sjögren's syndrome ⁵² ; decreased CD8⁺ T‐cell immunity to EBV in MS ⁵³ , ⁵⁴ ; improvement of MS with rituximab, which kills EBV‐infected B cells and uninfected B cells ⁵⁵ ; and a beneficial effect of EBV‐specific T‐cell therapy in MS. ⁵⁶ , ⁵⁷

Evidence for a role of EBV in the pathogenesis of BD

At present, the evidence for a role of EBV infection in the pathogenesis of BD is limited. Gotlieb‐Stematsky and colleagues found that the geometric mean titre of serum antibodies against EBV viral capsid antigen in 27 patients with primary affective disorders (depression and mania) was significantly higher than in 25 neurological disease controls (54.43 versus 16.47, respectively, P < 0.001). ⁵⁸ Another study found a direct correlation between the severity of depression in depressed patients and the titres of serum antibodies against EBV early antigen, but not viral capsid antigen. ⁵⁹ Haeri and colleagues found serological evidence of EBV reactivation more frequently in 100 pregnant women with depression than in 100 healthy pregnant women without depression ⁶⁰ ; this was interpreted by the authors as possibly being due to increased stress from the depression, but an alternative explanation is that the depression was caused by poorly controlled EBV infection. The high anti‐EBV antibody titres in BD might reflect a high number of EBV‐infected cells. It is interesting to note that clozapine, an atypical antipsychotic drug used to treat BD, inhibits EBV reactivation. ⁶¹

Evidence for a role of CD8⁺ T‐cell deficiency in BD

A key component of the EBV‐infected autoreactive B‐cell hypothesis of autoimmunity is that the accumulation of EBV‐infected autoreactive B cells in the target organ is attributed to a genetic defect in the ability of EBV‐specific CD8⁺ T cells to control EBV infection. ⁴ , ⁴² The most likely explanation for the defect is the CD8⁺ T‐cell deficiency that is a characteristic feature of the human chronic autoimmune diseases, including MS. ⁴² , ⁶² This CD8⁺ T‐cell deficiency is also present in the healthy blood relatives of patients with chronic autoimmune diseases, indicating that the deficiency is genetically determined. ⁴² In MS, it has been shown that the CD8⁺ T‐cell deficiency is due to a decrease in both the CD8⁺ effector memory (EM) T cells and the CD8⁺ EM re‐expressing CD45RA (EMRA) T cells. ⁶³

It is therefore of great interest that patients with BD have a decreased frequency of circulating CD8⁺ T cells ⁷ , ⁶⁴ and that the decrease in CD8⁺ T cells is due to a decrease in both CD8⁺ EM T cells and CD8⁺ EMRA T cells and is the most marked during the manic phase of bipolar disorder. ⁷ Moreover, the decrease in CD8⁺ EMRA T cells was found to be directly correlated with decreased fractional anisotropy, indicating decreased connectivity, in the corpus callosum and corona radiata. ⁷ It is likely that the deficiency of CD8⁺ T cells in BD impairs immune control of EBV, as it does in MS. ⁵⁴ , ⁶⁵

Benefit of sunlight

Seasonal affective disorder is characterised by recurrent episodes of major depression in autumn and winter with spontaneous remission in spring and summer. ⁶⁶ Most individuals with seasonal affective disorder also have BD. ⁶⁶ Bright artificial light, particularly in the early morning, is effective in reducing winter depressive symptoms of seasonal affective disorder. ⁶⁷ Bright light therapy is also efficacious in bipolar depression. ⁶⁸ Furthermore, exposure to morning sunlight reduces depression in seasonal affective disorder ⁶⁹ and shortens the duration of hospitalisation for bipolar depression. ⁷⁰ The mechanism for the beneficial effect of phototherapy on depression is unclear but has been variously attributed to effects on circadian rhythm, melatonin secretion or serotonin uptake. ⁷¹

Another possible mechanism whereby phototherapy (sunlight or artificial light) might be beneficial in BD is a light‐induced increase in the number of CD8⁺ T cells that are available to regulate EBV infection. Treatment in a solarium or exposure to natural sunlight increases the proportion of circulating CD8⁺ T cells and decreases the CD4/CD8 T‐cell ratio. ⁷² , ⁷³ , ⁷⁴ This effect of light is likely to be mediated at least partly by vitamin D for the following reasons: (1) within the immune system, the cells expressing the greatest concentration of the vitamin D receptor are activated CD8⁺ T cells ⁷⁵ ; (2) vitamin D augments the mitogen‐induced proliferation of CD8⁺ T cells and lowers the CD4/CD8 ratio in vitro ⁷⁶ ; (3) administration of vitamin D increases the CD8⁺ T‐cell count ⁷⁷ ; and (4) in vitamin D deficiency, the proportion of CD8⁺ T cells in the blood decreases and the CD4/CD8 ratio increases. ⁷⁸ Interestingly, T cells also have an intrinsic sensitivity to blue light, which increases T‐cell motility through a H₂O₂ signalling pathway. ⁷⁹

Role of stress

Stress in the form of negative life events is strongly associated with increases in the severity of subsequent depression and mania. ⁸⁰ It is important to note that stress suppresses the normal function of the immune system. Psychological stress compromises the ability of CD8⁺ T cells to prevent the reactivation of latent herpes simplex virus type I infection. ⁸¹ Furthermore, stress decreases the ability of CD8⁺ T cells to kill EBV‐infected B cells, ⁸² thereby inducing EBV reactivation. ⁸² , ⁸³ , ⁸⁴ Thus, stress might increase the number of EBV‐infected autoreactive B cells and plasma cells and therefore exacerbate BD, leading to increased stress, further suppression of CD8⁺ T‐cell control of EBV infection, increased EBV‐infected autoreactive B cells and plasma cells and further worsening of BD – a vicious circle, indeed.

Proposed hypothesis for the development of BD

It is proposed that the genes predisposing to BD can be subdivided into genes causing a general susceptibility to autoimmunity and genes causing specific susceptibility to BD. It is also postulated that the general susceptibility to autoimmunity is due to a genetic defect in the generation of the CD8⁺ T cells needed to regulate EBV infection. ⁴² Based on the EBV‐infected autoreactive B‐cell hypothesis of autoimmunity, ⁴ , ⁴² it is proposed that the following sequence of events leads to the development of BD (Figure 2). EBV infection in people with a genetic defect in the generation of CD8⁺ T cells and a general predisposition to autoimmunity results in a high number of EBV‐infected autoreactive B cells, which infiltrate lymphoid and non‐lymphoid organs, including the CNS. In those people who also carry genes conferring specific susceptibility to BD, autoreactive T cells specific for CNS components, such as the NMDA receptor, are activated by cross‐reacting foreign antigens or modified autoantigens. These brain‐reactive T cells then enter the CNS where they receive costimulatory survival signals from the EBV‐infected autoreactive B cells lodged in the CNS. In turn, the now‐surviving autoreactive T cells provide T‐cell help to the EBV‐infected autoreactive B cells, which then differentiate into plasma cells producing pathogenic autoantibodies, including anti‐NMDA receptor antibodies. The autoantibodies interfere with glutamatergic or other neurotransmission in the CNS, thereby giving rise to the symptoms of BD.

Figure 2.

Proposed role of EBV infection in the development of BD. EBV infects autoreactive naïve B cells in the tonsil, driving them to proliferate and then enter germinal centres. In the germinal centres, they again proliferate and differentiate into latently infected autoreactive memory B cells (Step 1), which exit from the tonsil and circulate in the blood (Step 2). The number of EBV‐infected B cells is normally controlled by EBV‐specific cytotoxic CD8⁺ T cells, which kill the infected B cells. However, if there is a defective CD8⁺ T‐cell control of EBV infection, EBV‐infected cells survive and proliferate, resulting in an increased number of EBV‐infected autoreactive memory B cells that enter the brain where they lodge (Step 3). Circulating autoreactive T cells that have been activated in peripheral lymphoid organs enter the brain where they are reactivated by EBV‐infected autoreactive B cells presenting target cell peptides (Tp) bound to major histocompatibility complex (MHC) molecules (Step 4). These EBV‐infected B cells provide costimulatory survival signals (B7) to the CD28 receptor on the autoreactive T cells and therefore inhibit the activation‐induced T‐cell apoptosis, which normally occurs when autoreactive T cells enter the brain and interact with non‐professional antigen‐presenting cells (APC) that do not express B7 costimulatory molecules ⁹² , ⁹³ (Step 6). After the autoreactive T cells have been reactivated by EBV‐infected autoreactive B cells, they produce cytokines such as interleukin‐2 (IL‐2), interferon‐γ (IFN‐γ) and tumour necrosis factor (TNF) and orchestrate an autoimmune attack on the target cells, such as neurons expressing the NMDA receptor (Step 5). The autoreactive T cells also provide T‐cell help to the EBV‐infected autoreactive B cells that then differentiate into plasma cells. These plasma cells produce pathogenic autoantibodies, which attack target cell components, such as NMDA receptors. BCR, B‐cell receptor; TCR, T‐cell receptor. Modified from Pender ⁴² through a Creative Commons Licence.

Testing the hypothesis

Is EBV infection a prerequisite for the development of BD?

Patients with MS and patients with systemic lupus erythematosus are almost universally (99–100%) seropositive for EBV. ⁸⁵ , ⁸⁶ To determine whether EBV infection is a prerequisite for the development of BD, serological studies should be conducted in individuals with BD and, for comparison, healthy subjects. It is important that IgG reactivity to both EBNA1 and EBV viral capsid antigen is tested because some EBV‐infected individuals may be seronegative for one of these EBV antigens.

Do EBV‐infected autoreactive B cells and plasma cells accumulate in the brain in BD?

Firstly, it should be determined whether there is an increased frequency of EBV‐infected B cells and plasma cells in the brain in BD compared with non‐autoimmune CNS disorders. Secondly, whether EBV‐infected B cells and plasma cells in the brain in BD are autoreactive could be addressed by determining whether they bind biotinylated NMDA receptor or other autoantigens in the same way that EBV‐infected plasma cells in the synovium bind citrullinated fibrinogen in rheumatoid arthritis. ⁵¹

Is the CD8⁺ T‐cell response to EBV decreased in BD?

Whether the CD8⁺ T‐cell response to EBV is decreased in BD could be addressed by immunological studies of the response of peripheral blood CD8⁺ T cells to EBV antigens in individuals with BD and in EBV‐seropositive healthy individuals, as have been used to demonstrate defective CD8⁺ T‐cell control of EBV infection in MS. ⁵⁴ It is also important to determine whether the CD8⁺ T‐cell deficiency in individuals with BD is also present in their healthy first‐degree relatives.

Does the benefit of phototherapy in BD correlate with improved CD8⁺ T‐cell control of EBV?

To determine whether the clinical benefit of phototherapy in BD is related to improved CD8⁺ T‐cell control of EBV infection, the clinical effect of exposure to sunlight or artificial bright light could be correlated with any changes in the numbers of total CD8⁺ T cells, CD8⁺ EM T cells and CD8⁺ EMRA T cells and with any changes in the CD8⁺ T‐cell response to EBV after phototherapy. In the case of artificial light, it will be important to ensure that the light includes the UVB wavelengths (280–320 nm) needed to stimulate vitamin D synthesis in the skin.

Does EBV vaccination protect against BD?

Epstein–Barr virus‐induced infectious mononucleosis, but not asymptomatic EBV infection, in healthy EBV‐seronegative young adults is effectively prevented by vaccination with recombinant EBV gp350 glycoprotein. ⁸⁷ The subjects who were vaccinated developed anti‐gp350 antibodies, which most likely provided the protective effect because monoclonal anti‐gp350 antibody neutralises EBV infectivity of B cells. ⁸⁸ The efficacy of this vaccine is limited because it protects only B cells, and not epithelial cells, from infection. Recently, it has been shown that vaccination of mice and non‐human primates with components of the glycoprotein gH/gL complex of the EBV viral‐fusion machinery potently neutralises infection of both epithelial cells and B cells. ⁸⁹ If BD truly is an EBV‐dependent chronic autoimmune disease, effective vaccination against EBV in early childhood should prevent BD.

Does BD respond to therapy aimed at controlling EBV infection?

If BD is an EBV‐induced chronic autoimmune disease, there are two ways to control EBV infection specifically and therefore treat BD: (1) treatment with antiviral drugs; and (2) improving immunity to EBV. Aciclovir and related drugs have a limited therapeutic effect in EBV‐induced diseases because they inhibit EBV only in the lytic phase when it utilises its own DNA polymerase in order to replicate its DNA. They are ineffective in the latent phase when EBV employs EBNA1 to hijack host cell DNA polymerase to replicate its DNA. In the future, drugs targeting specific EBV proteins, such as small‐molecule inhibitors of EBNA1 DNA binding, ⁹⁰ have the potential to be more effective in controlling EBV infection.

In order to improve CD8⁺ T‐cell immunity to EBV, individuals with BD could be treated by intravenously infusing EBV‐specific cytotoxic CD8⁺ T cells after expansion in vitro, as in the treatment of patients with MS. ⁵⁶ , ⁵⁷ A potential risk of EBV‐specific T‐cell therapy is that, if EBV‐infected cells do accumulate in the brain in BD, the transferred T cells might increase inflammation in the brain and cause clinical deterioration. If BD is induced by EBV infection, EBV‐specific T‐cell therapy has the potential to be curative. Another way of improving immunity to EBV is EBV vaccination. An effective EBV vaccine, when it becomes available, might be beneficial even in patients with established BD. Vaccination against EBV also has the potential to increase inflammation in the brain and cause clinical deterioration.

Conclusion

In conclusion, I have here proposed the novel hypothesis that BD is a chronic autoimmune disease caused by EBV infection of autoreactive B cells, which accumulate in the brain where they facilitate an autoimmune attack on brain components such as the NMDA receptor. It is postulated that the accumulation of EBV‐infected autoreactive B cells in the brain is a consequence of a genetically determined defect in the ability of CD8⁺ T cells to control EBV infection. According to the hypothesis, BD should be able to be treated by EBV‐specific T‐cell therapy and to be prevented by vaccination against EBV early in life. Exposure to sunlight or appropriate artificial light should also be beneficial in BD by augmenting CD8⁺ T‐cell control of EBV infection.

Conflict of interest

The author declares no conflict of interest.